Human Choline acetyltransferase(ChAT)ELISA Kit

Описание

Detection range of the kit is: 10 pmol/L -200 pmol/L

 

The kit is for the quantitative level of ChAT in the sample, adopt purified Human ChAT to coat microtiter plate, make
solid-phase antibody, then add ChAT to wells, Combine ChAT antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of ChAT in the samples is then determined by comparing the O.D. of the samples
to the standard curve.

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