Описание
Quantity size: 200ug/ml
Each vialcontains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Blocking peptide available forcompetition studies,sc-13186 P, (100 µg peptide in 0.5ml PBS containing < 0.1%sodiumazide and 0.2%BSA).
Source: MEL-1A-R is an affinity purified goat polyclonal antibodyraised against a peptidemapping near the C-terminus of MEL-1A-R of rat origin.
Background:
Melatonin (Mel), a hormone secreted by the pineal gland, is expressed at night in response to the circadian clock (1,2). Melatonin is thought to be involved in regulating reproductive physiological development and the progression of sexual maturation, and it is also thought to play a role in tumorigenesis (1,3). The melatonin receptors, MEL-1A-R and MEL-1B-R, are members of the super-family of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors (4,5). Signaling through the melatonin receptors inhibits adenylate cyclase and stimulates phospholipase Cb upon activation of pertussis toxin (PTX)-sensitive G proteins (4,5). MEL-1A-R may be involved in pacing the biological clock (5). However, both MEL-1A-R and MEL-1B-R are implicated in controlling cellular growth in response to melatonin (6,7).
Storage:
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
Application:
MEL-1A-R (R-18) isrecommended for detection of MEL-1A-R of mouse,rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml ofcell lysate)], immunofluorescence (starting dilution 1:50,dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30,dilution range 1:30-1:3000).
REFERENCES:
1. S.Huang, H., et al. 2005. Modulation bymelatonin of glutamatergicsynaptic transmission in the carp retina. J. Physiol. 569: 857-871.
2. Zeng, Q., et al. 2008. Exacerbatedmechanical allodynia in ratswith depression-like behavior. Brain Res. 1200: 27-38
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